Enter any desired search criteria in the specimen search section and click find specimens. Evaluation of the cobas taqman mtb test for the detection of. Applied biosystems taqman realtime pcr assays consist of targetspecific primers and one or more probes optimized for specific applications, including gene expression, snp genotyping, mirna, mutation detection, and copy number variation analysis. Comparison of the xpert mtbrif and cobas taqman mtb assays. The test is used to measure changes in hiv1 rna levels. Several commercial tests have been introduced during the past few years, for example, the cobas amplicor m. Evaluation of the cobas taqman mtb test for direct. Pdf comparison of the xpert mtbrif and cobas taqman mtb. The cobas ampliprepcobas taqman hcv test is an in vitro nucleic acid amplification test for the quantitation of hepatitis c viral hcv rna in human plasma or serum of hcvinfected individuals using the cobas ampliprep instrument for automated specimen processing and the. Clinical evaluation of cobas taqman pcr for the detection. Cobas ampliprepcobas taqman hiv1 qualitative test 2016 frontier science foundation 4 may 2016 11. We performed a prospective study to evaluate the diagnostic performance of the cobas taqman mtb test system. The cobas taqman mtb test, based on realtime pcr technology, was evaluated for direct.
The test is a nucleic acid amplification of hiv type 1, rna in human plasma. Mtb dna in clinical specimens are commercially available, e. Amplicor respiratory specimen preparation kit was used for dna extraction from clinical specimens. Cobas taqman 48 analyzer specifications instrument specifications technology the cobas taqman 48 analyzer is a fully automated amplification and detection system for nucleic acids using 5 nuclease technology. Retain testing of the complaint kits met specifications. Conversion to the cobas ampliprepcobas taqman cmv test. It uses taqman hydrolysis probes and primers that bind to a highly conserved and specific region of the 16s rrna sequence.
L aliquots of the decontaminated samples using the cobas taqman mtb test roche diagnostics, basel, switzerland, a realtime pcr assay targeting part of the 16s rrna gene for detection of mtb complex dna, according to the manufacturers instructions. We compared the sensitivities and specificities of peptide nucleic acid pna probebased realtime pcr pnaqpcr tbntm and cobas taqman mtb assays for detection of mycobacterium tuberculosis complex mtbc in clinical specimens. Sensitivity and specificity of cobas taqman mtb realtime polymerase chain reaction for cultureproven mycobacterium tuberculosis. Pdf the roche cobas amplicor mtb assay, recently replaced by the roche cobas. The cobas taqman mtb assay cobas assay roche diagnostics, basel, switzerland is one of the most widely used realtime pcr assays unitaid, 2015. Evaluation of the cobas taqman mtb test for the detection. Realtime pcr nucleic acid amplification and hydrolysis probes for the qualitative detection of mtb complex dna in human respiratory specimens. The advansure tbntm realtime pcr and cobas taqman mtb pcr assays were performed according to the manufacturers recommendations by using the slan realtime pcr detection system lg life sciences and cobas taqman 48 analyzer roche diagnostics, respectively.
Comparison of xpert mtbrif with probetec et dtb and cobas. We performed a prospective study in two hospitals in taiwan on 586 respiratory specimens. An automated test developed to test hiv in a single multiplex assay. No related items previous abstract next abstract 25 broadway.
Comparison of the xpert mtbrif and cobas taqman mtb. Pdf evaluation of cobas taqman mtb pcr for detection of. Cytological and virological medium performance and stability assessment using the cobas 4800 hpv test roche diagnostics used in france. To evaluate the performance of the cobas taqman mtb assay cobas. Performance evaluation of the cobas taqman mtb assay on. In this prospective study, 320 consecutive respiratory. However, there are few data comparing the xpert assay to the cobas taqman mtb test cobas. Comparison of the xpert mtbrif and cobas taqman mtb assays for detection of mycobacterium tuberculosis in respiratory specimens. Abbott molecular platforms for automated sample preparation m2000sp, left and. Cobas taqman mtb ctm test is a realtime pcr assay that amplifies part of the 16s rrna gene with the use of a taqman probe for the detection of mtb complex dna in clinical specimens. A total of 425 clinical specimens including 360 respiratory specimens and 65 nonrespiratory specimens were evaluated for comparative analysis. The mtb pcr assay was performed with 100l aliquots of the decontaminated samples using the cobas taqman mtb test roche diagnostics, basel, switzerland, a real.
Quest diagnostics will offer roches cobas ampliprepcobas taqman hiv1 test for hiv viral load testing under a multiyear agreement announced this week. Diagnostic molecular mycobacteriology in regions with low. As both the cobas ampliprepcobas taqman hiv1 test, v2. There are, however, limited studies validating its performance.
Rapid detection of mycobacterium tuberculosis using a. Software amplilink software is a windowsbased, liscompatible user interface that manages up to 3 cobas taqman 48 analyzers. A total of 1,093 samples from 446 patients, including 118 acidfast smearpositive and 975 acidfast smearnegative specimens, were investigated. A total of 1,093 samples from 446 patients, including 118 acidfast smearpositive and. The performance of the xpert assay has been evaluated in various clinical settings. The cobas taqman mtb test cobas mtb test roche diagnostics, basel, switzerland is one of the most widely used realtime pcr assays. Cobas ampliprepcobas taqman hcv test package insert.
Rapid molecular assays for detection of tuberculosis. The roche cobas taqman mtb ctm assay which focuses on a segment of the 16s rrna gene 19 is one of the most widely used commercial naats and it has attained 96% sensitivity and 74% specificity. Evaluation of the cobas taqman mtb test for direct detection of. Reagents cobas ampliprep cobas taqman hiv1 test pn. Evaluation of the cobas taqman mtb realtime pcr assay for. The cobas taqman mtb test cobas mtb test roche diagnostics, basel, switzerland is one of the most widely. Intended use this test is an in vitro nucleic acid amplification test for the qualitative detection of mycobacteria tuberculosis mtb complex dna in liquefied, decontaminated and concentrated human respiratory specimens, including sputum and bronchial alveolar lavages bal. Evaluation of cobas taqman mtb pcr for detection of mycobacterium tuberculosis article pdf available in journal of clinical microbiology 491.
Pdf nucleic acidbased amplification tests allow the rapid detection of mycobacterium tuberculosis. Pcr mixes were prepared, the dna was added and tubes were placed into the cobas taqman 48 analyzer for realtime pcr. Comparison of the genedia mtb detection kit and the cobas taqman mtb assay for detection of mycobacterium tuberculosis in respiratory specimens. Cobas taqman 48 analyzer specifications instrument. To evaluate the performance of the cobas taqman mtb assay cobas assay. The specimens found grid loads with specimens that match the search criteria and have the cobas. The document revision information section is located at the end of this document. Development of a high sensitivity taqmanbased pcr assay. Evaluation of cobas taqman mtb for direct detection of the. It uses real time pcr to detect hiv type 1 group m. The cobas assay uses primers and taqman hydrolysis probes that bind to specific regions with highly. Table 3 evaluation of the cobas taqman mtb test performance using. Diagnostic cultures performed with 7h11 agar, lowensteinjensen medium, and the. However, there are few data comparing the xpert assay to the cobas taqman mtb test roche diagnostics, basel, switzerland, one of the most widely utilized molecular assays for m.
Pdf evaluation of cobasr taqman mtb for direct detection of. Comparison of advansure tbntm pcr and cobas taqman. The cobas taqman mtb pcr test showed higher sensitivity for detection of the m. The cobas taqman mtb test, based on realtime pcr technology, was evaluated for direct detection of mycobacterium tuberculosis complex mtbc in respiratory specimens. Evaluation of cobas taqman mtb pcr for detection of. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. The test utilizes the amplicor respiratory specimen preparation kit for manual specimen preparation and the cobas.
However, the performance of naa tests according to clinical. Nucleic acidbased amplification tests allow the rapid detection of mycobacterium tuberculosis. However, there are few data comparing the xpert assay to the cobas taqman mtb test roche diagnostics, basel, switzerland, one of the most widely utilized. Cobas ampliprepcobas taqman hcv quantitative test, v2. By using culture as the reference method, the sensitivity and specificity of the cobas taqman mtb assay were. Cobas taqman mtb the qualitative cobas taqman mtb taqman mtb.
Manufacturer specifications cobas taqman 48 analyzer. Comparison of pna probebased realtime pcr and cobas. Roche diagnostics, tokyo, japan test has also been introduced to replace the wellestablished cobas amplicor assay. Sensitivity and specificity of cobas taqman mtb realtime. Tuberculosis is a global public health problem involving significant morbidity, and early diagnosis followed by adequate treatment is essential for the prevention of. Manufacturer specifications cobas taqman 48 analyzer, roche diagnostics please note. Molecular diagnostics realtime pcr virology womens. Testing of clinical plasma specimens by the cobas ampliprepcobas taqman cmv test capctm cmv, cobas amplicor cmv monitor test cam cmv, and a laboratorydeveloped assay using analytespecific reagents lc cmv demonstrated substantial result bias for capctm cmv versus cam cmv. We performed a direct headtohead comparison of xpert mtbrif with two widely used commercial assays, probetec et dtb dtb bectondickinson and cobas taqman mtb ctmmtb roche. The cobas taqman mtb assay is a realtime pcr qpcr kit for rapid detection of mycobacterium tuberculosis from clinical specimens. Clinical usefulness of routine afb culture and mtb pcr of. Cobas taqman mtb ctm test is a realtime pcr assay that amplifies part of the 16s rrna gene with the use of a taqman probe for the detection of mtb complex. Fully automated real time pcr analyzer that uses a closed system tubes, making it practical and easy to integrate into your laboratory daily routine.
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